The DUTCH Advanced Test provides the most complete picture of hormone health, offering detailed insights into oestrogen, progesterone, testosterone, cortisol, and their metabolites. This comprehensive test goes beyond standard hormone panels, measuring critical pathways involved in hormone production, detoxification, and clearance. It evaluates your body’s ability to break down and eliminate oestrogens, detect potential hormone imbalances, and assess the impact of stress on adrenal function. This test is ideal for those concerned about hormone-related symptoms like mood swings, fatigue, weight changes, or hormone-sensitive conditions such as PCOS, endometriosis, and oestrogen dominance.
With the DUTCH Advanced Test, you gain a deeper understanding of how your hormones impact your overall well-being. It not only measures hormone levels but also assesses how your body metabolises and clears them, providing critical information for personalised hormone support.
You can view a demo of our results page here to get a feel for the level of information we provide as standard.
This comprehensive assessment of oestrogen levels and metabolism, measures key oestrogen forms (oestradiol, oestrone, and oestriol) along with their breakdown pathways. It helps identify hormonal imbalances and assess oestrogen clearance, providing insights into hormone-related symptoms like PMS, perimenopause, weight gain, and breast health.
This dried urine test measures:
The most biologically active form of oestrogen, crucial for reproductive health, bone strength, and mood regulation.
A weaker form of oestrogen primarily produced in adipose tissue, often elevated in postmenopausal women.
The least potent form of oestrogen, mainly produced during pregnancy and considered more protective against oestrogen dominance.
The ratio of E3 to (E1 + E2), indicating the balance between protective and proliferative oestrogens.
A protective oestrogen metabolite, associated with lower breast cancer risk and reduced DNA damage.
A less active oestrogen metabolite that is typically protective but needs efficient methylation to avoid toxic build-up.
A potentially harmful oestrogen metabolite linked to DNA damage and cancer risk if not efficiently cleared.
Another potentially carcinogenic oestrogen metabolite that can damage DNA if not properly detoxified.
A more stimulatory oestrogen metabolite that promotes cell growth and can increase the risk of oestrogen-sensitive cancers.
The ratio reflecting the balance between protective and stimulatory oestrogen metabolism, often used to assess cancer risk.
A methylated form of oestrogen that is protective and associated with reduced cancer risk.
A methylated form of 2-OH oestrone, promoting safer oestrogen metabolism and reducing cancer risk.
The ratio reflecting how effectively 2-OH oestrogens are being methylated for safe excretion.
A methylated form of 4-OH oestradiol, helping reduce the toxic effects of this potentially harmful metabolite.
The methylated form of 4-OH oestrone, reducing the carcinogenic potential of this metabolite.
The ratio reflecting the efficiency of 4-OH oestrone methylation, important for reducing cancer risk.
The ratio reflecting the efficiency of 4-OH oestradiol methylation, reducing the potential for DNA damage and cancer risk.
This test evaluates progesterone metabolism, providing insights into overall hormone balance, adrenal health, and the body’s ability to convert progesterone into its active metabolites. It can help identify issues related to stress resilience, mood regulation, menstrual cycle stability, and overall hormone function.
This dried urine test measures:
The primary urinary metabolite of progesterone, reflecting overall progesterone production and metabolism.
A powerful neurosteroid derived from progesterone, known for its calming, anti-anxiety effects on the brain.
A downstream metabolite of allopregnanolone, reflecting how well progesterone is being converted into neuroprotective compounds.
An intermediate in the conversion of progesterone to allopregnanolone, with potential calming and mood-regulating effects.
Another progesterone metabolite involved in the formation of neurosteroids and regulation of mood and anxiety.
A precursor to aldosterone and cortisol, involved in blood pressure regulation and electrolyte balance.
An adrenal steroid involved in the production of cortisol and regulation of stress response and immune function.
The ratio of progesterone to estradiol, reflecting the balance between these two critical sex hormones, important for cycle regulation and mood stability.
This test measures primary androgens, including DHEA, testosterone, and their metabolites, providing a comprehensive view of androgen production and metabolism. It helps assess energy, muscle mass, libido, mood stability, and overall vitality, while also highlighting potential imbalances related to adrenal function and hormone conversion pathways.
This dried urine test measures:
A precursor hormone involved in the production of testosterone, oestrogen, and other androgens, and linked to energy, mood, and muscle mass.
A form of DHEA that provides a more stable measure of adrenal function and androgen status.
A direct precursor to testosterone and oestrone, playing a key role in muscle growth, libido, and energy levels.
A DHT metabolite that reflects 5α-reductase activity and androgen metabolism.
A DHEA metabolite formed through 5β-reductase pathways, often associated with inflammation and immune activation.
The primary male sex hormone, critical for muscle mass, libido, energy, and mood in both men and women.
An inactive testosterone isomer, used to assess testosterone metabolism and adrenal function.
A potent androgen derived from testosterone, responsible for male characteristics, hair loss, and prostate growth.
A downstream metabolite of DHT, reflecting the conversion of testosterone to more potent androgens, often linked to hair loss, acne, and prostate health.
Reflects the body’s production of DHEA, a critical precursor for testosterone, oestrogen, and other androgens, supporting energy, mood, and stress resilience.
Measures the enzyme activity that converts testosterone to the more potent DHT, which influences hair growth, prostate health, and androgenic effects.
The balance between active testosterone and its inactive isomer, often used to assess androgen status and detect testosterone supplementation in athletes.
DUTCH testing offers a comprehensive assessment of adrenal function by measuring free and metabolised cortisol, cortisone, and related glucocorticoids across a full diurnal cycle. This advanced urine-based test provides insights into both hormone production and clearance, helping to identify patterns of adrenal fatigue, stress response, and HPA axis imbalance.
This dried urine test measures:
Measures cortisol levels at their expected peak, reflecting adrenal function and the cortisol awakening response (CAR).
Assesses cortisol levels as they decline from their morning peak, reflecting the body’s stress response and energy availability.
Indicates cortisol levels in the later part of the day, reflecting the body’s ability to sustain energy, focus, and stress tolerance.
Measures cortisol when levels should be lowest to allow for melatonin production and restful sleep.
The combined daily output of active cortisol, reflecting overall adrenal function and stress response.
The total amount of inactive cortisone produced throughout the day, reflecting the body’s ability to inactivate cortisol for recovery.
The balance between active cortisol and inactive cortisone, indicating the efficiency of 11β-HSD enzyme activity and overall stress response.
A primary cortisol metabolite, reflecting total cortisol clearance and adrenal output.
A primary cortisone metabolite, reflecting cortisone clearance and the overall inactivation of cortisol.
The sum of THF and THE, representing the total metabolised glucocorticoids and overall adrenal function.
Measures inactive cortisone levels at their natural peak, reflecting overnight adrenal output and the body’s ability to clear active cortisol.
Reflects cortisone levels as they decline from the morning peak, indicating adrenal function and cortisol metabolism during the active part of the day.
Cortisone – Afternoon
Assesses cortisone levels at the end of the day, when they should be lowest to allow for melatonin production and restful sleep.
This test measures urinary melatonin levels throughout the day, providing insight into your body’s natural sleep-wake cycle and overall circadian rhythm function. It helps identify potential imbalances that may impact sleep quality, energy levels, and overall wellbeing.
This dried urine test measures:
Measures melatonin levels in the morning, reflecting overnight production and the body’s ability to suppress melatonin with morning light.
Assesses melatonin during the day, which should be low to support alertness and daytime energy.
Measures melatonin in the afternoon, which remains low under normal circadian rhythm to allow for daytime focus.
Reflects melatonin production in the evening, when it should rise to promote sleep onset and restful sleep.
This test assesses exposure to common endocrine disruptors, including heavy metals, plastics, and industrial chemicals, which can interfere with hormone function and disrupt metabolic processes. It provides insights into potential toxic burden, helping guide personalised detoxification and lifestyle recommendations.
This dried urine test measures:
A common plastic chemical that can mimic oestrogen, potentially disrupting hormone balance and fertility.
A group of industrial chemicals linked to hormone disruption, immune dysfunction, and cancer risk.
A persistent environmental toxin associated with thyroid disruption, immune dysfunction, and reproductive issues.
Another persistent pollutant found in non-stick and waterproof products, linked to hormone disruption and immune stress.
A common metal toxin that can accumulate in the brain, bones, and lungs, potentially contributing to neurotoxicity and bone weakness.
A heavy metal that can interfere with cellular energy production and increase the risk of cancer, heart disease, and diabetes.
A toxic metal that can affect kidney function, bone health, and respiratory function, often from smoking or industrial exposure.
An essential trace element in small amounts but toxic at high levels, potentially disrupting blood sugar regulation.
A highly toxic heavy metal known for its impact on brain development, nervous system function, and cardiovascular health.
A potent neurotoxin that can impair brain function, immune health, and detoxification pathways.
A common industrial metal that can cause skin irritation, respiratory issues, and allergic reactions if levels are too high.
This panel includes a range of nutritional organic acids that reflect vitamin status, neurotransmitter metabolism, and cellular energy production. It provides insights into nutrient deficiencies, neurotransmitter imbalances, and potential mitochondrial dysfunction, supporting a comprehensive understanding of overall metabolic health.
This dried urine test measures:
A marker of vitamin B6 deficiency and tryptophan metabolism, associated with inflammation and immune dysfunction.
A byproduct of leucine metabolism, often elevated in biotin deficiency or protein breakdown.
An indicator of vitamin B12 status, elevated when B12 is deficient or methylation is impaired.
This is a breakdown product of dopamine, a brain chemical that supports mood, focus, and motivation. Abnormal levels may reflect changes in dopamine activity or nervous system stress.
A norepinephrine and epinephrine metabolite, reflecting sympathetic nervous system activity and adrenal stress.
A tryptophan metabolite that reflects immune activation and neuroinflammation.
A neurotoxic tryptophan metabolite linked to inflammation, neurodegeneration, and mood disorders.
A marker of DNA oxidative damage, reflecting cellular stress and free radical exposure.
A marker of glutathione status, reflecting the body’s detox capacity and antioxidant defenses.
A serotonin metabolite reflecting gut serotonin production and intestinal health.
Urinary creatinine testing provides insights into kidney function, hydration status, and overall muscle health, helping to standardise hormone metabolite measurements for more accurate hormone assessment.
This dried urine test measures:
An overall measure of muscle metabolism and kidney function from the entire collection period.
Reflects overnight muscle metabolism and kidney function during the body’s fasted state.
Reflects muscle breakdown and kidney function during the active part of the day.
Measures muscle and kidney clearance later in the day, reflecting activity level and hydration status.
Indicates muscle metabolism and renal function during the winding down phase of the day.
Your test kit and all instructions are posted directly to you, and there is no need to visit a collection centre.
Collect your urine samples at 4 set times during the day.
Mail your sample back to the lab using the prepaid envelope and packaging provided.
Results for this test typically available in 2-3 weeks and will be published in your online dashboard.
